Abstract: Inclusion conditions and inclusion ratio of parsley seed essential oil (PEO) with β-cyclodextrin (CD) or its derivatives including HP-β-CD and DM-β-CD were investigated at the molecular level by UV spectroscopy. The inclusion constants and thermodynamic parameters including ?G, ?H and ?S in the range of 25—55 ℃ were also determined. Solid inclusion complexes were prepared by saturated aqueous solution method and identified by UV spectroscopy and integrating sphere technique. Meanwhile their stability was evaluated as well. The results showed that the inclusion ratio between β-CD and parsley seed essential oil was 1:1. The inclusion capacities of β-CD was in the deceasing order: HP-β-CD ＞ DM-β-CD ＞ β-CD. The inclusion process was an exothermic and enthalpy-driven process. The inclusion complexes had good stability to light and heat.

Key words: parsley seed essential oil, β-cyclodextrin and its derivatives, inclusion reaction, inclusion complex, integrating sphere