Optimization of Enzymatic Extraction Process for Collagen from Grass Carp Bladders by Response Surface Methodology

Abstract

Abstract: The pepsin-catalyzed hydrolysis of grass carp bladders was optimized by response surface methodology to improve the extraction yield of collagen. We explored the effects of hydrolysis parameters, enzyme loading, hydrolysis time and liquid-to-solid ratio on the extraction yield of collagen. Their optimal values for collagen extraction were found to be 40 mg/g, 48 h and 80:1 (mL/g), respectively. Under the optimized conditions, the actual extraction yield of collagen was 17.82%, which was close to the predicted value (18.04%). The results of analysis of variance showed that the fitted regression model was reliable, and each hydrolysis parameters had a significant effect on the extraction yield of collagen.

Key words: response surface methodology, pepsin, grass carp bladders, collagen

CLC Number:

References

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