Abstract: A direct competitive chemiluminescene enzyme immunoassay (dcCL-EIA) was developed for the rapid determination of fumonisin B1 in cereal samples. Assay parameters including coating antigen (FB1-OVA) and enzyme labeled anti-FB1 antibodies and reaction time were optimized to be 0.4 μg/mL, 3000-fold dilution and 20 min, respectively. The analytical sensitivity of the developed method was 0.13 ng/mL, and the IC50, intra-assay and inter-assay coefficients of variation were 1.43 ng/mL, 2.4% and 9.2%, respectively. The recovery rates for FB1 in negative corn samples at three levels were in the range of 100.2%～115.4%. A total of 40 cereal samples were screened by DC-CLEIA, ELISA kit and HPLC, respectively. The results indicated that the correlation coefficients (R2) for the results from ELISA kit and HPLC were 0.9793 and 0.9851, respectively. The dcCL-EIA method is sensitive, rapid and accurate, and is suitable for the large-scale screening of FB1 in cereal samples.

Key words: fumonisin B1, chemiluminescene enzyme immunoassay, direct competitive ELISA