Determination of Lutein Stereoisomers by HPLC

Abstract

Abstract: An HPLC-DAD-atmospheric pressure chemical ionization (APCI)-MS method was proposed to quantify and qualify lutein stereoisomers. A C30 column was found to be more effective in separating lutein stereoisomers than a C18 column. The mobile phase was composed of methanol and deionized water (98:2, V/V). A good relationship between peak area and injection amount was found in the range of 40 to 200 ng. The proposed method was accurate, reproducible, economical and suitable for the determination of lutein stereoisomers.

Key words: lutein, stereoisomers, high performance liquid chromatography (HPLC)

CLC Number:

TS201.2

References

[1] Roberts R L, Green J, Lewis B. Lutein and zeaxanthin in eye and skin health[J]. Clinics in Dermatology, 2009, 27(2): 195-201.
[2] Ma Le, Lin Xiaoming. Effects of lutein and zeaxanthin on aspects of eye health[J]. Journal of the Science of Food and Agriculture, 2010, 90(1): 2-12.
[3] 惠伯棣. 类胡萝卜素化学及生物化学[M]. 北京: 中国轻工业出版社, 2005: 248-255.
[4] 朱海霞, 郑建仙. 叶黄素（Lutein）的结构、分布、理化性质及生理功能[J]. 中国食品添加剂, 2005, (5): 48-55.
[5] 李大婧, 刘春泉, 白云峰, 等. 叶黄素、玉米黄质研究进展-叶黄素、玉米黄质的结构、性质和生物学功能[J]. 核农学报, 2006, 20(1): 64-67.
[6] B?hm V, Puspitasari-Nienaber N L, Ferruzzi M G, et al. Trolox equivalent antioxidant capacity of different geometrical isomers of α-carotene, β-carotene, lycopene, and zeaxanthin[J]. Journal of Agricultural and Food Chemistry, 2002, 50(1): 221-226.
[7] 惠伯棣, 张西, 文镜. 反相C30柱在HPLC分析类胡萝卜素中的应用[J]. 食品科学, 2005, 26(1): 264-270.
[8] 惠伯棣, 李京, 裴凌鹏. 应用C30-HPLC-PDA分离与鉴定番茄红素几何异构体[J]. 食品工业科技, 2006, 27(7): 49-54.
[9] 惠伯棣, 李京, 裴凌鹏, 等. C30-HPLC-PDA分离与鉴定β, β-胡萝卜素几何异构体[J]. 食品科学, 2006, 27(10): 252-255.
[10] 张艳, 惠伯棣, 裴凌鹏, 等. C30柱分离万寿菊花中的叶黄素类化合物初探[J]. 食品科学, 2006, 27(11): 424-428.
[11] Lee H S, Castle W S, Coates G A. High-performance liquid chromatography for the characterization of carotenoids in the new sweet orange (Earlygold) grown in Florida, USA[J]. Journal of Chromatography A, 2001, 913(1): 371-377.
[12] Emenhiser C, Sander L C, Schwartz S J. Capability of a polymeric C30 stationary phase to resolve cis-trans carotenoid isomers in reversed-phase liquid chromatography[J]. Journal of Chromatography A, 1995, 707(2): 205-216.
[13] Liu S C, Lin J T, Yang D J. Determination of cis- and trans-α and β-carotenoids in Taiwanese sweet potatoes (Ipomoeabatatas (L.)Lam.) harvested at various times[J]. Food Chemistry, 2009, 116(3): 605-610.
[14] Aman R, Biehl J, Carle R, et al. Application of HPLC coupled with DAD, APCI-MS and NMR to the analysis of lutein and zeaxanthin stereoisomers in thermally processed vegetables[J]. Food Chemistry, 2005, 92(4): 753-763.
[15] Chen B H, Peng H Y, Chen H E. Changes of carotenoids, color, and vitamin A contents during processing of carrot juice[J]. Journal of Agricultural and Food Chemistry, 1995, 43: 1912-1918.