Determination of Danofloxacin Mesylate in Animal Liver by Flow Injection Chemiluminescence

Abstract

Abstract: A new method for the rapid determination of danofloxacin mesylate was proposed based on the fact that the chemiluminescence (CL) reaction between luminol and potassium periodate is sensitized by danofloxacin mesylate in alkaline solution. Samples were extracted with phosphate buffer solution and the extract was cleaned up using a solid-phase extraction column and determined by flow-injection chemiluminescence as described for the standard curve preparation. Under optimal conditions, an excellent linear relationship between chemiluminescence intensity and danofloxacin mesylate concentration was in the range of 4.53 × 10-6–2.27 × 10-3 mg/mL. The limit of detection for danofloxacin mesylate was 3.76 × 10-6 mg/mL with a relative standard deviation (RSD) of 1.9% (n = 11). The average recovery rates of danofloxacin mesylate when spiked to pork and chicken liver were in the range of 69.00%–97.50% (n = 5).

Key words: danofloxacin mesylate, flow injection chemiluminescence, animal liver, solid phase extraction

CLC Number:

ＧＡＯ xiang-yang. Determination of Danofloxacin Mesylate in Animal Liver by Flow Injection Chemiluminescence[J]. FOOD SCIENCE, 2012, 33(22): 246-249.

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