FOOD SCIENCE

Previous Articles     Next Articles

PCR-DGGE Analysis of Microbial Diversity of Homemade Pickles in Sichuan Region

ZHANG Xian-qin,ZHANG Xiao-ping,AO Xiao-lin,LIU Xiao-qian,PU Biao   

  1. 1. College of Resource and Environmental Science, Sichuan Agricultural University, Chengdu 611130, China;
    2. College of Food Science, Sichuan Agricultural University, Ya’an 625014, China
  • Online:2013-06-25 Published:2013-06-17

Abstract:

The microbial diversity of 20 homemade pickles in Sichuan region was analyzed by PCR-DGGE. Meanwhile,
representative stripes were selected to be sequenced for construction of a phylogentic tree based on their sequences.
The results revealed abundant bacteria species but a few fungi in the pickles. The genus Lactobacillus was the dominant
populations while uncultured bacteria were sub-dominant populations, which accounted for 56% and 32% of the total
bacteria species, respectively. Lactobacillus plantarum played an important role in pickle fermentation. The closely related
species of Meyerozyma guilliermondii and Kodamaea ohmeri were the predominant fungi in Sichuan homemade pickles.

Key words: polymerase chain reaction-denatured gradient gelel ectrophoresis(PCR)-DGGE, pickle, microbial diversity, bacteria, fungi