Simultaneous Determination of Adenosine and Cordycepin in Cordyceps militaris (L.) by High Performance Lquid Chromatography

Abstract

Abstract:

In this paper, an HPLC method for determining the contents of adenosine and cordycepin in Cordyceps militaris
(L.) is described. The optimum conditions for extraction of adenosine and cordycepin were determined as pure water, 40
min, 30 ℃, 700 W and 1 for extraction solvent, time, temperature, ultrasonic power and extraction number, respectively. The
separation column was an XBridgeTM C18 (5 μm, 4.6 mm × 250 mm), and the column temperature was 30 ℃. The mobile
phase was composed of pure water and methanol (84:16, V/V) at a flow rate of 1.0 mL/min. The sample loading volume was
10 μL. UV detection was performed at 254 nm. The average recoveries from spiked blank and real samples were 99.13% and
95.78% for adenosine, and 99.18% and 94.35% for cordycepin, respectively. This method is characteristics of simplicity,
and high sensitivity and accuracy.

Key words: high performance liquid chromatography (HPLC), Cordyceps militaris (L.), adenosine, cordycepin

YUAN Mi，LE Xin，XU Lu-rong，ZHI Yue-e，ZHOU Pei. Simultaneous Determination of Adenosine and Cordycepin in Cordyceps militaris (L.) by High Performance Lquid Chromatography[J]. FOOD SCIENCE.

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Simultaneous Determination of Adenosine and Cordycepin in Cordyceps militaris (L.) by High Performance Lquid Chromatography

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