Abstract:

A competitive ELISA kit for fast detection of streptomycin (SM) residues in royal Jelly was developed.
SM complete antigens were prepared and monoclonal antibodies against streptomycin were produced by
hybridoma technology and in vivo ascites induction method. Results showed that the kit’s Logit/Log calibration
curve was y = －2.1x ＋ 0.8(correlation coefficient, r = 0.9943), with linear range of 4 to 324 μg/kg. The half inhibitory
concentration (IC50) was 79 μg/kg, sensitivity was 0.1 μg/kg, and limit of detection was 4 μg/kg. The detection accuracy
was over 90%. The recoveries of SM from spiked positive samples were between 67.7% and 86.7% and the coefficients
of variation for replicate measurements ranged from 4.3% to 5.6%. The developed ELISA kit can be applied for the
quantitative detection of SM residues in royal jelly.

Key words: royal jelly, streptomycin, monoclonal antibodies, competitive ELISA