Fabrication and Analytical Application of Three-Dimensional Paper-Based Microfluidics for Rapid Detection of Nitrite

doi:10.7506/spkx1002-6630-201322069

Abstract

Abstract:

Medium-speed qualitative filter paper was employed to fabricate paper-based microfluidics and 3D paper-based
microfluidics were integrated by wax printing-bookbinding method; a nitrite indicator containing 63.4 g/L citric Acid, 8.61 g/L sulfanilamide
and 2.59 g/L N-(1-naphthyl) ethylenediamine coupled with a lab-prepared colorimetric detection device was used
for nitrite determination. The 3D paper-based microfluidics could rapidly test nitrite, and the image acquired was analyzed
using a gray model for gray intensity representing the concentration of nitrite. The linear range was 0–10 mg/L, with correlation
coefficient of 0.9920. The detection limit of the method was 2 mg/L, and the recovery of nitrite from spiked samples
was 91.4%–102.0%. After stored under normal conditions and 4 ℃ for 7 weeks, the color intensity of the paper-based microfluidics
for nitrite detection were little change. The 3D paper-based microfluidics can provide a simple, easy to use, low-cost
and sensitive approach for rapid detection of in foods with small sample consumption.

Key words: paper-based microfluidics, three-dimension, nitrite, rapid detection

CLC Number:

XIAO Liang-pin，LIU Xian-ming，LIU Qi-shun，ZHONG Run-tao，ZHOU Xiao-mian，LIN Bing-cheng，DU Yu-guang. Fabrication and Analytical Application of Three-Dimensional Paper-Based Microfluidics for Rapid Detection of Nitrite[J]. FOOD SCIENCE, 2013, 34(22): 341-345.

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