Abstract: In this paper, the effect of small black soybean whey protein, which was treated at different tempreture, on S180 animal model was studied. The results indicated that there was no tumefaction of the pancreas of 75℃ treated whey protein at 3 g/kg and 10 g/kg intake doses, and the inhibition effect on S180 tumor and tumor index of whey protein treated at 75℃ were better than 65℃(P＜0.05) significantly. The thymus index of mice in 75℃whey protein group,which was at the same level as the basic group ,was significantly higher than those of mice in other groups(P＜0.05). The white blood cell, the ear swelling degree and HC50 of mice in 75℃ whey protein group were significantly higher than those of mice in the model group(P＜0.01) and the 65℃ whey protein group (P＜0.01).Therefore, the antitumor ability on S180 tumor can be significantly improved by small black soybean whey protein treated at 75℃.