Purification of the bacteriocin produced by Lactobacillus plantarum KLDS1.0391

Abstract

Abstract: The purpose of this study is to purify the bacteriocin produced by Lb. plantarum KLDS1.0391. The bacteriocin was separated by chromatography techniques and the molecular mass of purified bacteriocin was determined by mass spectrometric analysis. The optimal method of purification was finally determined as follow: Cell-free supernatants of Lb. plantarum KLDS1.0391 was precipitated by 70% ammonium sulphate. Then the precipitates were dissolved in 1/40 volume of 0.05 M sodium acetate (pH 6.5), followed by cation exchange chromatography (using COLUMN XK 16/20 chromatographic column filled with SP Sepharose fast flow), and reverse-phase chromatography (using SOURCE 15RPC ST 4.6/100 further purify the solution for two times). The purity of the purified samples was determined by high-performance liquid chromatography (HPLC), and the molecular mass of samples was estimated on Tricine-SDS-PAGE, which concluded that the molecular mass was approximately 2000 kDa. Matrix-assisted laser desorption ionization–time of flight (MALDI-TOF) MS analysis of purified bacteriocin revealed that the molecular mass was approximately 1770 kDa.

CLC Number:

TS252.1

References

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