Comparison of Methods for DNA Extraction from Soybean for Detection of Genetically Modified Organism

Abstract

Abstract:

The objective of this study was to screen a suitable method for DNA extraction from soybean by comparison of five generally applied DNA extraction methods. After extraction, the quality of soybean DNA was examined with ultraviolet spectrophotometry, agarose gel electrophoresis and real-time fluorescent PCR, respectively. The results showed that the DNA extracted by Bayer method contained many PCR inhibitors; the purity and yield of DNA extracted by Monsanto method did not meet the demand of the quality standard of DNA in molecular experiment; DuPont method, Qiagen kit and Tiangen kit could extract high-quality DNA which was suitable for real-time fluorescent PCR, but Qiagen kit cost too much. These evidences indicated that DuPont method and Tiangen kit were suitable methods to extract DNA from soybean for detection of genetically modified ingredients.

CLC Number:

S565.1

References

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