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Influences of Plant Hormones on the Natamycin Production of Streptomyces natalensis

WEI Bao-dong, WANG Li-yan, YU Si-wen, PAN Ya-hui   

  1. College of Food Science, Shenyang Agricultural University, Shenyang 110866, China
  • Online:2014-06-15 Published:2014-07-03

Abstract:

Screening of five plant hormones was carried out to find out those that can effectively enhance natamycin
production in shake-flask culture by Streptomyces natalensis upon addition to the medium. A mathematical model was built
using Box-Behnken experimental model and used to determine the optimal concentrations of selected plant hormones in the
medium. The results showed that indole-3-acetic acid, gibberellin and 6-benzyl amino purine effectively increased natamycin
production. At optimized concentrations of 5.5, 14.8 and 20.5 mg/L for these plant hormones, respectively, the yield of
natamycin was 1.805 g/L, which was 1.63 times higher than that of the control group.

Key words: plant hormones, Streptomyces natalensis natamycin, fermentation, response surface methodology