Synthesis of Artificial Antigen and Preparation of Polyclonal Antiserum against Metronidazole

doi:10.7506/spkx1002-6630-201419038

Abstract

Abstract:

Specific high-titer mouse polyclonal antisera against metronidazole were prepared by converting the hydroxyl
groups in the metronidazole molecule into carboxyl groups using succinic anhydride method and conjugating the modified
molecule (MNZ) respectively with albumin from bovine serum (BSA) and ovalbumin (OVA) using carbodiimide method
to form the immunogen MNZ-BSA and the coating antigen MNZ-OVA. UV spectroscopy and SDS-PAGE confirmed that
MNZ was coupled successfully to the carrier protein. Specific high-titer polyclonal antisera were obtained by immunizing
Balb/c mice with MNZ-BSA at a dose of 20 μg or 50 μg for each mouse for 8 weeks, respectively, and the titers were
achieved to be 1:104 and IC50 was 61.39 ng/mL.

Key words: metronidazole, artificial antigen, polyclonal antibody, rapid detection

CLC Number:

S859.84

LIU Ru-biao, HU Xiao-fei, ZHANG Gai-ping, WANG Fang-yu, DENG Rui-guang, LIU Lu, JIA Guo-chao, LIU Xi. Synthesis of Artificial Antigen and Preparation of Polyclonal Antiserum against Metronidazole[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201419038.

[1]	TIAN Jingsheng, LI Dongdong, ZHAO Lingyu, GAO Wenhui. Preparation and Application of Molecularly Imprinted Electrochemical Sensor for Rapid Detection of Quinoxaline Drug Residues [J]. FOOD SCIENCE, 2021, 42(6): 316-324.
[2]	LIU Na, CAO Qiang, XIAO Yushi, HUANG Rong, CHENG Lei, LIU Huan, HAN Gang, LI Jincheng, LI Menglong, WU Lidong. Recent Progress of Sensors Based on Dopamine and Its Derivatives in Food Safety Rapid Detection [J]. FOOD SCIENCE, 2021, 42(5): 332-339.
[3]	HUANG Dongwei, GU Guizhang, HU Kena, GAO Xingjie, ZHANG Jinjie, YANG Wenge, XU Dalun. Detection of Guanine in Fish Flesh by Protein Adsorption onto Macroporous Resin Combined with Surface-Enhanced Raman Spectroscopy [J]. FOOD SCIENCE, 2021, 42(18): 298-305.
[4]	YANG Jianfei, MA Qian, ZUO Yong, HUANG Xueqin, XU Jia, WANG Dingli, ZHOU Yaojin, YANG Keyi. A Spectrophotometric Reagent for the Rapid Detection of Nitrite and Its Application to Pickles [J]. FOOD SCIENCE, 2021, 42(18): 321-328.
[5]	ZHANG Biao, HUANG Na, ZHANG Wanli, WANG Shuo, SHENG Wei. Fluorescence Immunoassay Combined with Magnetic Separation Using Upconversion Nanoparticles as Label for the Detection of Tyramine in Food Samples [J]. FOOD SCIENCE, 2021, 42(14): 270-275.
[6]	WANG Zouluqi, LI Lihuang, LI Danyang, AI Chaochao, REN Lei, SUN Benqiang. Construction of Down-conversion Fluorescence-Aptamer Immunochromatographic Strip for Rapid Detection of Aflatoxin B1 [J]. FOOD SCIENCE, 2021, 42(12): 295-301.
[7]	LU Jie, TIAN Jing, LIANG Zhenhua, WANG Jinmei, KANG Wenyi, MA Changyang, LI Changqin. Application of Near Infrared Spectroscopy in the Rapid Detection of Total Sugar Content in Lentinula edodes [J]. FOOD SCIENCE, 2021, 42(12): 189-194.
[8]	Fan Yiling, WANG Shujuan, LI Qiongqiong, HU Ying, SONG Minghui, Qin Feng, Liu Hao, YANG Meicheng. Rapid Detection of Shiga Toxin-producing Escherichia coli by Recombinase Polymerase Amplification Combined with Centrifugal Compact Disc Microfluidic Chip [J]. FOOD SCIENCE, 2021, 42(10): 297-304.
[9]	WANG Changchang, MA Liang, LIU Wei, GUO Ting, TAN Hongxia, ZHANG Yuhao, ZHOU Hongyuan, CHEN Lu, LI Daoliang. Advances in Aptasensors Based on Smart Materials for Rapid Detection of Mycotoxins [J]. FOOD SCIENCE, 2020, 41(3): 305-313.
[10]	WU Yuhan, CHEN Wei. Rapid Detection of Tetracycline and Penicillin in Milk by Lateral Immunochromatography [J]. FOOD SCIENCE, 2020, 41(24): 281-286.
[11]	LIU Ruobing, HAO Yihuan, YANG Xi, JIAO Wenya, WANG Xianghong. A Fluorescence Method Based on AccuBlue and Nucleic Acid Aptamer for Detection of Enrofloxacin in Animal-Derived Foods [J]. FOOD SCIENCE, 2020, 41(24): 310-315.
[12]	JIN Dengpeng, ZHOU Xiong, LIU Huan, YANG Jiao, HUANG Zhuoquan, BU Lingling, KE Qianhua, LIU Chunhong. Proteomics Analysis of Breast Milk Whey Based on Immunoaffinity Chromatography and ProteoMiner [J]. FOOD SCIENCE, 2020, 41(24): 54-60.
[13]	XIN Liang, ZHANG Lanwei. Recent Progress in Nucleic Acid-Microfluidic Chips Used for Detection of Foodborne Pathogens: A Review [J]. FOOD SCIENCE, 2020, 41(23): 266-272.
[14]	LIU Xiya, TIAN Chunmei, ZHENG Lufei, ZHONG Pingsheng, ZHANG Xinfang, REN Jiali. Preparation of Magnetic Three-Dimensional Reduced Graphene and Its Application in Electrochemical Immunoassay of Mycobacterium tuberculosis H37Ra in Milk [J]. FOOD SCIENCE, 2020, 41(22): 308-314.
[15]	SUN Zongbao, LIANG Liming, YAN Xiaojing, ZOU Xiaobo, WANG Tianzhen, LIU Xiaoyu, LI Junkui. Detection of Freshness Indexes of Imported Chilled Beef Using Hyperspectral Imaging Technology [J]. FOOD SCIENCE, 2020, 41(22): 315-323.

Synthesis of Artificial Antigen and Preparation of Polyclonal Antiserum against Metronidazole

RichHTML

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments