Development of a Multiplex PCR-DHPLC Detection Method for Five Staphylococcal Enterotoxin Genes

doi:10.7506/spkx1002-6630-201424047

FOOD SCIENCE

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Development of a Multiplex PCR-DHPLC Detection Method for Five Staphylococcal Enterotoxin Genes

CHEN Bin1,2, ZHENG Jing1,2, WANG Ying1,3, HUANG Xiao-rong1,2, LIN Jie1,2, PENG Hua-yi1,2, SHAO Bi-ying1,2,*

1. Fujian Entry-Exit Inspection and Quarantine Bureau, Fuzhou 350001, China;
2. Fujian Provincial Key Laboratory of Inspection and Quarantine Technology Research, Fuzhou 350001, China;
3. College of Food Sciences, Fujian Agriculture and Forestry University, Fuzhou 350002, China

Online:2014-12-25 Published:2014-12-29
Contact: SHAO Bi-ying

Abstract

Abstract:

Primer pairs specific for the five staphylococcal enterotoxin genes SEA, SEB, SEC, SED, and SEE were designed
and synthesized. Using multiplex polymerase chain reaction coupled with denaturing high performance liquid chromatograph
(MPCR-DHPLC), a detection method for these five staphylococcal enterotoxin genes were established after the polymerase
chain reaction (PCR) reaction conditions were optimized. The sensitivity and specificity of the detection method were
then determined. The results showed that the quintuplex PCR-DHPLC method could detect specifically staphylococcal
enterotoxin genes with detection limit of 100 CFU/mL. The MPCR-DHPLC method is useful for the rapid, accurate and
high-throughput detection of staphylococcal enterotoxins in food samples.

Key words: Staphylococcus aureus, enterotoxin, MPCR, DHPLC

CLC Number:

Q93.332

CHEN Bin1,2, ZHENG Jing1,2, WANG Ying1,3, HUANG Xiao-rong1,2, LIN Jie1,2, PENG Hua-yi1,2, SHAO Bi-ying1,2,*. Development of a Multiplex PCR-DHPLC Detection Method for Five Staphylococcal Enterotoxin Genes[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201424047.

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Development of a Multiplex PCR-DHPLC Detection Method for Five Staphylococcal Enterotoxin Genes

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