Abstract:

Objective: To construct inter-simple sequence repeat (ISSR) DNA fingerprinting database of Saccharomyces cerevisiae
and to establish corresponding specific sequence characterized amplified region (SCAR) markers for use in the classification,
identification and analysis of the genetic relationship among S. cerevisiae strains and patent protection. Methods: On the basis of
ISSR DNA fingerprinting analysis, 23 Saccharomyces cerevisiae strains were clustered by UPGMA and their classification and
identification were conducted. Then two specific ISSR bands from strains 9 and 15 were converted into two SCAR markers for
rapid strain identification. Results: The 23 strains could be divided into three categories. Of these, category I included strains 1, 2, 4, 7, 15,
16, 17, 19, 20, 21 and 23, category II included strains 10, 11, 12, 13, 14 and 18 in which strains 10 and 11 were the same, and category Ⅲ
included strains 3, 5, 6, 8, 9 and 22 which belonged to the same strain with a similarity coefficient of 0.85. Furthermore, two specific bands
were converted into SCAR marker for strain identification. Conclusion: The development of ISSR fingerprints and SCAR markers for
rapid identification of Saccharomyces cerevisiae strains is significant for industrial production because there is no significant difference in
genetic background of Saccharomyces cerevisiae strains and it is often occurs that the same strains are called different names.

Key words: Saccharomyces cerevisiae, inter-simple sequence repeat (ISSR), DNA fingerprint, sequence characterized amplified region (SCAR)