Optimization of Extraction Conditions for Yam Storage Proteins through Alkali-Extraction and Acid-Precipitation by Response Surface Methodology

doi:10.7506/spkx1002-6630-201516002

Abstract

Abstract:

The extraction of storage proteins from ‘Baiyu’ yam tubers grown in Jiaozuo, Henan province was optimized
using combination of one-factor-at-a-time method, Box-Behnken design (BBD) and response surface methodology (RSM).
Three factors influencing the extraction yield of yam storage proteins, i.e., pH, liquid/material ratio and extraction time
were investigated. The results showed that all these three factors had a notable effect on the extraction yield of yam storage
proteins and their significance could be ranked in a decreasing order of pH > liquid/material ratio > extraction time. The
optimal extraction conditions were determined as 8.5, 3:1 (mL/g) and 20 min for pH, liquid/material ratio and extraction
time, respectively. The predicted extraction yield of yam storage proteins under the optimized conditions was 57.88%,
agreeing with the experimental value (57.74%). The regression model predicting the extraction of yam storage proteins was
highly significant and had excellent goodness of fit, suggesting its usefulness.

Key words: yam storage proteins, response surface methodology, extraction process, optimization

CLC Number:

TS201.1

ZHAO Yue, GAO Qi, WANG Xiaowen, JIA Youfeng, ZHANG Junwei, AI Xinzi, XUE Youlin. Optimization of Extraction Conditions for Yam Storage Proteins through Alkali-Extraction and Acid-Precipitation by Response Surface Methodology[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201516002.

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Optimization of Extraction Conditions for Yam Storage Proteins through Alkali-Extraction and Acid-Precipitation by Response Surface Methodology

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