Multilocus Sequence Typing of Listeria monocytogenes Isolated from Imported and Exported Animal-Derived Foods

doi:10.7506/spkx1002-6630-201612038

FOOD SCIENCE

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Multilocus Sequence Typing of Listeria monocytogenes Isolated from Imported and Exported Animal-Derived Foods

LIU Erlong, YUAN Muyun, DENG Jianying, XING Fang, LÜ Yingzi, JIANG Yuan, XUE Feng, SHAO Jingdong, XU Longyan,

1. Huangpu Entry-Exit Inspection and Quarantine Bureau, Guangzhou 510730, China;
2. Guangdong Entry-Exit Inspection and Quarantine Bureau, Guangzhou 510623, China;
3. Guangzhou Panyu Quality and Technology Supervision and Testing Institute, Guangzhou 511400, China;
4. Jiangsu Entry-Exit Inspection and Quarantine Bureau, Nanjing 210001, Ch

Online:2016-06-25 Published:2016-06-29
Contact: XU Longyan E-mail:xlyciq@126.com

Abstract

Abstract:

This study was devised to understand the sequence types (STs) and the genetic and evolutionary relationship ofdifferent Listeria monocytogenes isolated from imported and exported animal-derived food samples. Seven house-keepinggenes were selected as target genes for PCR amplification. Sequencing, analysis and comparison of the 7 housekeepinggenes were conducted. Their alleles and STs codes were obtained, and their evolutionary relationships were analyzed byunweighted pair group method using arithmetic average (UPGMA). A total of 51 STs were obtained from 89 strains bymultilocus sequence typing (MLST), among which 26 were novel. The 5 most common STs were ST8 (9.0%), ST121(9.0%), ST7 (5.6%) and ST87 (5.6%) as well as the novel STnew 3 (7.8%). ST456, ST34, ST343, ST19, ST517, ST201,ST98, ST330 and ST73 were found for the first time in China. The evolutionary relationship analyzed by UPGMA methodshowed that the isolates were grouped into 3 clusters, which were consistent with three lineages classified by serotype ofL. monocytogenes. MLST is a useful method to evaluate genetic evolution. This method is applicable to study the populationstructure and epidemiological traceability of L. monocytogene isolates from imported and exported animal-derived food samples.

Key words: Listeria monocytogenes, mutilocus sequence typing (MLST), evolutionary relationship

CLC Number:

R377

LIU Erlong, YUAN Muyun, DENG Jianying, XING Fang, Lü Yingzi, JIANG Yuan, XUE Feng, SHAO Jingdong, XU Longyan,. Multilocus Sequence Typing of Listeria monocytogenes Isolated from Imported and Exported Animal-Derived Foods[J]. FOOD SCIENCE, doi: 10.7506/spkx1002-6630-201612038.

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Multilocus Sequence Typing of Listeria monocytogenes Isolated from Imported and Exported Animal-Derived Foods

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