FOOD SCIENCE ›› 2021, Vol. 42 ›› Issue (15): 159-166.doi: 10.7506/spkx1002-6630-20200914-171

• Nutrition & Hygiene • Previous Articles     Next Articles

Synthesis of Eriocitrin and Its Protective Effect on in Hydrogen Peroxide-Induced Oxidative Damage in Human Umbilical Vein Endothelial Cells

LIANG Zeng’enni, WANG Qiuan, ZHANG Juhua, SU Donglin, FU Fuhua, LI Gaoyang, LIU Wei, SHAN Yang   

  1. (1. Institute of Agricultural Product Processing, Hunan Academy of Agricultural Sciences, Changsha 410125, China; 2. Hunan Key Laboratory of Fruits & Vegetables Storage, Processing and Quality Safety, Changsha 410125, China;3. College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China)
  • Online:2021-08-15 Published:2021-08-27

Abstract: Eriocitrin was successfully synthesized by aluminum trichloride-pyridine demethylation reaction from hesperidin, as confirmed by 1H and 13C nuclear magnetic resonance (NMR), to explore its protective effect and mechanism on human umbilical vein endothelial cells (HUVEC) from hydrogen peroxide (H2O2)-induced oxidative damage. The results showed that eriocitrin had no toxic effect on cells HUVEC, and even significantly promoted the survival of HUVEC cells suffering from oxidative stress though without clear dose dependence (P < 0.05). Eriocitrin significantly reduced the levels of reactive oxygen species (ROS) and malondialdehyde (MDA) content (P < 0.05), increased antioxidant enzyme activities (superoxide dismutase, glutathion peroxidase and catalase) (P < 0.05), up-regulated Bcl-2/Bax protein ratio (P < 0.05) and down-regulated p53 protein expression (P < 0.05). It is suggested that eriocitrin can effectively prevent H2O2-caused damage to HUVEC cells, which is related to its antioxidant activity and inhibition on cell death.

Key words: eriocitrin; synthesis; oxidative damage; human umbilical vein endothelial cells

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