FOOD SCIENCE ›› 2023, Vol. 44 ›› Issue (24): 323-328.doi: 10.7506/spkx1002-6630-20230404-029

• Safety Detection • Previous Articles     Next Articles

Tailored Aptamer-Based Visual Colorimetric Detection of Staphylococcal Enterotoxin A Using Gold Nanoparticles

CUI Liwei, WEI Rong, CHANG Weidan, YUE Xiaoyu, XU Wentao   

  1. (1. School of Food and Biological Engineering, Henan University of Animal Husbandry and Economy, Zhengzhou 450046, China; 2. Key Laboratory of Precision Nutrition and Food Quality, Ministry of Education, Department of Nutrition and Health, China Agricultural University, Beijing 100191, China)
  • Online:2023-12-25 Published:2024-01-02

Abstract: Objective: To select and use aptamers as molecular recognition elements to construct a biosensor based on the salt effect of gold nanoparticles for the rapid quantitative detection of staphylococcal enterotoxin A (SEA). Methods: Molecular docking and molecular dynamics simulation were used to truncate the SEA aptamer and predict the results. The simulation results were verified colorimetrically using gold nanoparticles. The optimal SEA aptamer was used as the molecular recognition element. The reaction system was optimized, the relationship between SEA mass concentration and absorbance ratio was explored, and the accuracy, precision and specificity of the developed method were evaluated. Results: An SEA aptamer with short sequence, high affinity, strong specificity and stability was selected. This method was characterized by low limit of detection (LOD) and satisfactory recoveries for spiked samples. Conclusion: Molecular simulation can effectively improve the screening efficiency of aptamers, and the proposed method can be used for rapid detection of SEA.

Key words: staphylococcal enterotoxin A; visualization; aptamer; truncation; molecular dynamics simulation; gold nanoparticles-based colorimetry

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