Development of Insertion-Deletion Markers for Cabernet Sauvignon Grapes Based on Whole Genome Resequencing and Their Usefulness in Wine Authentication

doi:10.7506/spkx1002-6630-20250121-153

FOOD SCIENCE ›› 2025, Vol. 46 ›› Issue (18): 92-101.doi: 10.7506/spkx1002-6630-20250121-153

• Bioengineering • Previous Articles Next Articles

Development of Insertion-Deletion Markers for Cabernet Sauvignon Grapes Based on Whole Genome Resequencing and Their Usefulness in Wine Authentication

SUN Qian, LI Na, YANG Bingyi, WANG Jie, WANG Xiaonan, BO Wenhao, XING Ranran, CHENG Jinxin, ZHU Baoqing

(1. Academy of Investigation, China People’s Police University, Langfang 065000, China; 2. Forest Food and Health Research Institute, Beijing Key Laboratory of Forest Food Processing and Safety, Beijing Forestry University, Beijing 100083, China; 3. Chinese Academy of Inspection and Quarantine, Beijing 100176, China)

Online:2025-09-25 Published:2025-08-19

Abstract

Abstract: This study used genomic resequencing to screen for insertion-deletion (InDel) markers that enable the identification of Cabernet Sauvignon grapes and examined their application effectiveness in Cabernet Sauvignon wine authentication. The results showed that among 13 markers selected from the whole genome, 9 InDel markers (Del-1–6, In-1, and In-4–5) with polymorphism and good amplification effect were successfully developed and validated by polymerase chain reaction (PCR). Del-2–6, In-1, and In-5 were able to distinguish between Cabernet Sauvignon and Marceline grapes. It was found that Del-2, Del-4, Del-6 could be used to distinguish between Cabernet Sauvignon and Marceline wines. These results enrich the molecular markers of grapes, providing a theoretical basis for the application of InDel markers in genetic research such as grape resource genetic diversity, variety identification, and DNA fingerprinting.

Key words: insertion-deletion molecular marker, Cabernet Sauvignon grapes, variety identification, Cabernet Sauvignon wine, authentication

CLC Number:

S663.1

SUN Qian, LI Na, YANG Bingyi, WANG Jie, WANG Xiaonan, BO Wenhao, XING Ranran, CHENG Jinxin, ZHU Baoqing. Development of Insertion-Deletion Markers for Cabernet Sauvignon Grapes Based on Whole Genome Resequencing and Their Usefulness in Wine Authentication[J]. FOOD SCIENCE, 2025, 46(18): 92-101.

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Development of Insertion-Deletion Markers for Cabernet Sauvignon Grapes Based on Whole Genome Resequencing and Their Usefulness in Wine Authentication

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