Extraction of Ursolic Acid from Loquat (Eriobotrya japonica (Thunb.) Lindl.) Leaves

doi:10.7506/spkx1002-6300-201004007

FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (4): 28-34.doi: 10.7506/spkx1002-6300-201004007

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Extraction of Ursolic Acid from Loquat (Eriobotrya japonica (Thunb.) Lindl.) Leaves

JIAO Rong-rong1,2，SUN Yi-min1,2,*，SUN Ruo-qiong2,3，GENG Ji-hua4，SONG Wen-jia1,2，LUO Le1,2

1. Key Laboratory of Functional Molecular Solid of Anhui Province, Wuhu 241000, China；2. College of Chemistry and Materials
Science, Anhui Normal University, Wuhu 241000, China；3. College of Life Science, Anhui Normal University, Wuhu 241000,
China；4. Talent Natural Extract Co. Ltd., Wuhu 241007, China

Received:2009-04-07 Revised:2009-10-12 Online:2010-02-15 Published:2010-12-29
Contact: SUN Yi-min E-mail:mysun@mail.ahnu.edu.cn

Abstract

Abstract:

In this study, ultrasonic-assisted extraction of ursolic acid from loquat leaves was systematically investigated. The influence of five technological parameters including ethanol concentration, extraction time, liquid/solid ratio, extraction number and material particle size on ursolic acid yield was assessed by uniform design for obtaining optimal ultrasonic-assisted extraction process. Ursolic acid was quantified by HPLC method. The optimized process was validated by 20-fold pilot scale enlargement. The optimal values of technological parameters were obtained as follows: ethanol concentration 80% － 100%, extraction time 50－65 min or 80－100 min, and extraction number 1. Visual analysis demonstrated the complicated influence of liquid/solid ratio and material particle size on ursolic acid yield.

Key words: ursolic acid, uniform design method, HPLC, visual analysis method

CLC Number:

JIAO Rong-rong1,2，SUN Yi-min1,2,*，SUN Ruo-qiong2,3，GENG Ji-hua4，SONG Wen-jia1,2，LUO Le1,2. Extraction of Ursolic Acid from Loquat (Eriobotrya japonica (Thunb.) Lindl.) Leaves[J]. FOOD SCIENCE, 2010, 31(4): 28-34.

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Extraction of Ursolic Acid from Loquat (Eriobotrya japonica (Thunb.) Lindl.) Leaves

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