Isolation, Purification and Monosaccharide Analysis of a Neutral Polysaccharide from Polygonatum odoratum (Mill) Druce

doi:10.7506/spkx1002-6630-201015022

FOOD SCIENCE ›› 2010, Vol. 31 ›› Issue (15): 100-102.doi: 10.7506/spkx1002-6630-201015022

• Basic Research • Previous Articles Next Articles

Isolation, Purification and Monosaccharide Analysis of a Neutral Polysaccharide from Polygonatum odoratum (Mill) Druce

WANG Qiang1， LI Sheng-yu2，YANG Fan1，LIU Chun-ming1,*

1. Central Laboratory of Changchun Normal University, Changchun 130032, China；
2. Center of Agro-food Technology, Jilin Academy of Agricultural Sciences, Changchun 130033, China

Received:2009-12-14 Online:2010-08-15 Published:2010-12-29
Contact: LIU Chun-ming1,* E-mail:chunmingliu2000@yahoo.com.cn

Abstract

Abstract:

Crude polysaccharides were obtained from Polygonatum odoratum (Mill) Druce by water extraction and ethanol precipitation, and were sequentially purified by ion exchange chromatography on DEAE-cellulose column and gel permeation chromatography on Sepharose CL-6B column and a neutral polysaccharide fraction was obtained. Its structure was determined using HPLC, FTIR and UV spectroscopy. The results showed that the polysaccharide fraction was mainly composed of mannose and glucose at a molar ratio of 5:1 and also contained a small amount of galactose and that its molecular weight was approximately 1.21 × 106 D.

Key words: Polygonatum odoratum (Mill) Druce, polysaccharide, purification, monosaccharide composition

CLC Number:

R284.2

WANG Qiang1， LI Sheng-yu2，YANG Fan1，LIU Chun-ming1,*. Isolation, Purification and Monosaccharide Analysis of a Neutral Polysaccharide from Polygonatum odoratum (Mill) Druce[J]. FOOD SCIENCE, 2010, 31(15): 100-102.

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Isolation, Purification and Monosaccharide Analysis of a Neutral Polysaccharide from Polygonatum odoratum (Mill) Druce

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