Abstract:

For establishing an enzyme-linked immunosorbent assay (ELISA) method for rhodamine B determination, a complete antigen R123-BSA and a coating antigen R123-OVA were prepared by coupling rhodamine123 with the carrier proteins of bovine serum albumin (BSA) and ovalbumin (OVA) using glutaraldehyde method, respectively. The synthesis of rhodamine123 artificial antigens was confirmed by animal experiments. A novel anti-rhodamine123 polyclonal antibody was prepared, which was successfully applied to the determination of rhodamine B in food with a limit of detection of 0.001 ng/mL. The development of anti-rhodamine123 antibody will provide a valuable tool for the determination of rhodamine B in food seasoning products.

Key words: rhodamine 123, rhodamine B, glutaraldehyde method, artificial antigen, enzyme-linked immunosorbent assay (ELISA)