Abstract:

Response Surface Methodology was applied to optimize the fermentation of Aspergillus niger for cellulose. In the first step, two level factorial design of Plackett-Burman was used to evaluate the influence of seven related factors. The results showed that the effects of temperature, time and packing volume on the activities of cellulase were significant among the seven factors. In the follow step, the optimal conditions of the three factors were assessed by the RSA. The ptimal conditions of the three factors were as follows: fermentation temperature of 31.02 ℃, 73.17 h of fermentation and 100.4 ml of packing volume. Under the optimal condition, the enzyme activity was increased by 34.4 %, from 267.81 to 360.02 U/ml.

Key words: Aspergillus niger, cellulase, response surface, SAS system