FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (11): 131-133.doi: 10.7506/spkx1002-6630-200911028

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Antibacterial Activity and Emulsifying Activity of Polylysine and Dextran Conjugate by Maillard Reaction

XIE Jian-fei,YANG Yu-hong,CHEN Hong-man*,KAN Guo-shi   

  1. College of Biotechnology, Shenyang Agricultural University, Shenyang 110161, China
  • Received:2008-10-24 Revised:2009-02-03 Online:2009-06-01 Published:2010-12-29
  • Contact: XIE Jian-fei E-mail:jf-xie@126.com

Abstract:

A Maillard reaction was carried out between 1 g of polylysine (PL) produced through fermentation of Streptomyces albus and 9 g of dextran with 40 kD molecular weight. The covalent attachment of PL to dextran was confirmed by SDS-PAGE and the absorbance measurement of Maillard reaction system at 420 nm wavelength was conducted to determine the conjugation progress between the above two materials. Subsequently, the antibacterial activity and emulsifying activity of PL-dextran conjugate were investigated. The results indicated that the PL-dextran conjugate not only had higher emulsifying activity than that of 0.9% dextran or 0.1% PL, but retained the antibacterial activity of PL. Its emulsifying activity reached the maximum at pH 7.0, and did not decrease in the presence of 1.75 mol/L of NaCl.

Key words: PL-dextran, emulsifying activity, antibacterial activity

CLC Number: