Abstract:

Objective: To realize the status of Enterobacter sakazakii contamination in infant formula milk powder, and to study relevant contamination routes and rapid detection methods. Methods: Enterobacter sakazakii colored medium and VITEK automatic microbial biochemical analyzer were used in isolation and identification, PCR and fluorescence PCR were used for detection. Results: The detection rate of Enterobacter sakazakii is 1.56% in 192 infant formula milk powder product samples, 13.33% in 60 material samples and 0% in 30 environment samples in production, respectively. Conclusion: There are hidden safety questions in Fujian infant formula, and most contaminations are from material. Moreover, fluorescence PCR and colored medium can be used for rapid isolation and identification of Enterobacter sakazaki in infant formula milk powder.

Key words: infant formula milk powder, Enterobacter sakazakii, contamination, fluorescence PCR