Abstract:

A multi-residue analytical method for eight sulfonamides in fish muscle based on matrix solid-phase dispersion (MSPD) coupled with high performance liquid chromatography (HPLC) was developed. This method was environmentally friendly using ethyl acetate as the elution solvent. 0.5 g of fish muscle mixed with 2 g of C18 packing material was grounded and filled into a SPE column. After washing with hexane, sulfonamides were eluted with ethyl acetate and determined using RP-HPLC with UV detector at 270 nm. Sulfonamides were separated on C18 column using 50 mmol/L NaH2PO4-acetonitrile (70:30, V/V) as mobile phase at a flow rate of 0.7 ml/min, and the column temperature was 30 ℃. The linear range of this method is from 0.010 μg/ml to 1.000 μg/ml with the correlation coefficient of over 0.9999. The spike recoveries are in the range of 76.0% to 115.0% and the relative standard deviation (RSD) is less than 6.4% (n = 6). The limit of detection is 0.020 μg/g in a 0.5 g of sample.

Key words: HPLC, fish muscle, MSPD, low toxic solvent, sulfonamide residues