Sequence Analysis of Glutamate Gecarboxylase Gene and Flanking Regions from Streptococcus thermophilus Y-2

doi:10.7506/spkx1002-6630-201103029

FOOD SCIENCE ›› 2011, Vol. 32 ›› Issue (3 ): 121-125.doi: 10.7506/spkx1002-6630-201103029

• Bioengineering • Previous Articles Next Articles

Sequence Analysis of Glutamate Gecarboxylase Gene and Flanking Regions from Streptococcus thermophilus Y-2

LIN Qian，YANG Sheng-yuan，LU Zhao-xin，LU Feng-xia，BIE Xiao-mei，ZOU Xiao-kui

1. Department of Chemistry and Biology, Yulin Normal University, Yulin 537000, China；
2. Department of Biology, Hanshan Normal University, Chaozhou 521041, China；
3. College of Food Science and Technology, Nanjing Agricultural University, Nanjing 210095, China

Received:2010-06-04 Revised:2010-12-31 Online:2011-02-15 Published:2011-01-13

Abstract

Abstract: The glutamate decarboxylase gene (gadB) and its flanking regions from Streptococcus thermophilus Y-2 were investigated by homologous search in GenBank with Blastn and Blastx. There was no any other gene showing significant similarity with gadB, while the protein product of gadB had high similarity with several bacterial glutamate decarboxylases. Phylogenetic tree based on glutmamte decarboxylase sequences indicated that Fusobacterium nucleatum subsp. polymorphum ATCC 10593 showed the closest relationship with S. thermophilus Y-2. The upstream flanking region of gadB was homologous to bacterial transposases, while the downstream flanking region of gadB encoding a putative protein was similar to bacterial glutamate/γ-aminobutyric acid antiporters (GadC). The order of gadB and gadC in S. thermophilus Y-2 genome was similar to that of E. coli and Shigella flexneri, but different from that of Lactococcus lactis and Lactobacillus brevis. Insertion sequence IS1216 was present in genomes of S. thermophilus LMG 18311, CNRZ1066, and LMD-9, but not gadB or gadC.

Key words: Streptococcus thermophilus, glutamate decarboxylase, &gamma, -aminobutyric acid, sequence analysis

CLC Number:

LIN Qian，YANG Sheng-yuan，LU Zhao-xin，LU Feng-xia，BIE Xiao-mei，ZOU Xiao-kui. Sequence Analysis of Glutamate Gecarboxylase Gene and Flanking Regions from Streptococcus thermophilus Y-2[J]. FOOD SCIENCE, 2011, 32(3 ): 121-125.

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Sequence Analysis of Glutamate Gecarboxylase Gene and Flanking Regions from Streptococcus thermophilus Y-2

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