Abstract: Objective: To investigate the effect of dietary squid on lipids metabolism in fatty liver in rats. Methods: Twenty four male SD rats were randomly divided into 3 groups and control group. The control was fed AIN76 diet and model group AIN76 diet plus 1% orotic acid, while squid group AIN76 diet plus 1% orotic acid and 10% squid. After 10 days, serum triglyceride (TG), total cholesterol (TC)，and high density lipoprotein cholesterol (HDL-C) levels were determined. Hepatic lipid concentrations (TG, TC, PL) and the activities of malic enzyme (ME), glucose-6-phosphate dehydrogenase (G6PDH), fatty acid synthase (FAS), carnitin palmitoyl transferase (CPT), peroxisomal β-oxidation enzymes were also examined. Results: After fed orotic acid, liver TC and TG levels were significantly increased, so did the activity of FAS, ME and G6PDH, but the activity of CPT drop. Taking squid could reduce the liver TC and TG and the activity of FAS, ME, G6PDH but increased the activity of peroxisomal β-oxidation enzymes in orotic acid-induced fatty liver in rats. Conclusion: This study indicated that the dietary squid could inhibit liver lipids cumulating in orotic acid-induced fatty liver in rats by reducing the activity of FAS, ME and G6PDH and increasing the activity of peroxisomal β-oxidation enzymes.

Key words: squid, fatty liver, orotic acid, lipids metabolism, enzyme activity