Abstract:

Polysaccharides were extracted from different parts of Clematis songarica Bge. and determined by using spectrophotometry method with glucose as the standard. The optimal extraction parameters of polysaccharides were explored by orthogonal experiments to be extraction temperature of 90 ℃, material-liquid ratio of 1: 60, extraction time of 2.5 h and repeated extraction number of 5 for stem; extraction temperature of 90 ℃, material-liquid ratio of 1: 60, extraction time of 2 h and repeated extraction number of 5 for leaves; extraction temperature of 90 ℃, material-liquid ratio of 1: 60, extraction time of 2 h and repeated extraction number of 5 for flowers; extraction temperature of 90 ℃, material-liquid ratio of 1: 60, extraction time 2 h and repeated extraction number of 5 for fruits. The absorbance of polysaccharides extracted from stem, leaves, flowers and fruits at 600 nm were 0.408, 0.493, 0.418 and 0.315, respectively.

Key words: Clematis songarica Bge., polysaccharides, extraction processing, optimization