Abstract:

In order to establish a method for rapid determination of enrofloxacin, flow-injection chemiluminescence (FI-CL) system of luminol and potassium ferricyanide was developed. Results indicated that enrofloxacin exhibited a significantly enhanced effect on chemiluminescence reaction between luminol and potassium ferricyanide. An excellent linear range for enrofloxacin determination was 6 × 10-11－6 × 10-7 mol/L under the optimal condition. The detection limit of enrofloxacin was 2.7 × 10-13 mol/L and relative standard deviation for the determination was 1.2% (n = 11). A satisfactory analytical result was obtained during enrofloxacin determination using this developed method.

Key words: enrofloxacin, flow-injection chemiluminescence, luminol, potassium ferricyanide