FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (22 ): 188-191.doi: 10.7506/spkx1002-6300-200922042

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Preparation of Immunoaffinity Column for Separation and Indrect ELISA Determination of Gentamycin

LI Zhe,HU Yong-ming,ZHANG Liu-wei,LIU Li-qiang,CHEN Wei,WANG Li-bing,XU Chuan-lai*   

  1. (School of Food Science and Technology, Jiangnan University, Wuxi 214122, China)
  • Received:2009-07-08 Online:2009-11-15 Published:2010-12-29
  • Contact: XU Chuan-lai* E-mail:xcl@jiangnan.edu.cn

Abstract:

Gentamycin residues were analyzed through indrect ELISA method, and immunoaffinity columns specific to gentamycin was prepared for its separation. The optimal ELISA for detecting gentamycin in swine tissues was studied. The IC50 for gentamycin was 3.8 ng/ml. The linear range was 0.1~25 ng/ml (R2=0.99) with a detection limit of 0.1 ng/ml. The immunoaffinity column was prepared using such antibody, and could be eluted with 5ml methanol/PBS (7:3, V/V) solution. The rate of adsroption to gentamycin was more than 90% and recovery more than 60%, could be repeatedly used for about 5 times.

Key words: gentamycin, polyclonal antibody, indirect competitive-ELISA, immunoaffinity column

CLC Number: