Toxigenic Potential of Bacillus cereus in Row Milk

doi:10.7506/spkx1002-6300-200922053

FOOD SCIENCE ›› 2009, Vol. 30 ›› Issue (22 ): 236-239.doi: 10.7506/spkx1002-6300-200922053

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Toxigenic Potential of Bacillus cereus in Row Milk

YANG Yuan，CHEN Qing-sen*，WU Hai-qing

Tianjin Key Laboratory of Food Biotechnology, College of Biotechnology and Food Science,
Tianjin University of Commerce, Tianjin 300134, China

Received:2009-07-06 Online:2009-11-15 Published:2010-12-29
Contact: CHEN Qing-sen*， E-mail:chqsen@tjcu.edu.cn

Abstract

Abstract:

In order to evaluate the toxigenic potential of Bacillus cereus in row milk, 100 samples of row milk from 10 cities of China were screened, and Bacillus cereus were found in 33 (33%) samples with the concentration within 1×103～1×106 CFU/ ml. Using PCR the isolates were checked for the presence of the hblA gene of the hemolysin BL (HBL) complex and the bceT genes of the enterotoxin T. HBL was detected by blood plate. Totally 25 (75.8%) of the 33 B. cereus isolates produced the hemolysin BL, while 24 (72.7%) isolates possessed the bceT gene, and as a result, 31(93.9%) B. cereus isolates produced at least one toxin. The results suggest that it is necessary to monitor the Bacillus cereus contamination in row milk.

Key words: raw milk, Bacillus cereus, hemolysin BL, hblA gene, bceT gene

CLC Number:

TS201.3

YANG Yuan，CHEN Qing-sen*，WU Hai-qing. Toxigenic Potential of Bacillus cereus in Row Milk[J]. FOOD SCIENCE, 2009, 30(22 ): 236-239.

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Toxigenic Potential of Bacillus cereus in Row Milk

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