Abstract:  Polysaccharides were prepared from the leaves of Phyllostachys pubescens. The crude polysaccharides were extracted from the dried leaves by ultrasonic method and fractionated precipitation by 95% ethanol while the protein was removed with Savag’s method. After being further lyophilized, these polysaccharides were purified by DEAE-52 cellulose column chromatog-raphy and Sephadex glucan gel column chromatography, respectively. The results showed that Savag’s deprotein method can get rid of 97.2% protein with the yield of polysaccharide as high as 37.6% with 50% ethanol precipitation. The yield of polysaccharides is the highest by DEAE-52 cellulose column chromatography which can be 46.4% and the polysaccharides extracted by DEAE-52 cellulose column chromatography with twice water elution can be changed into neutral polysaccharides of white powder by G-75 column chromatography and G-100 column chromatography. Water and NaCl as elution solvent can lead to good purifying efficiency on polysaccharides.

Key words: Phyllostachys pubescens, polysaccharides from bamboo leaves, column chromatography, isolation, purification