Abstract: By high-performance liquid chromatography (HPLC), UV spectrum and high-performance lamina chromatography (HPTLC) and color characteristic reaction to quantify the flavonoids, the basic components of thick celery extractions were identified as apigenin based on the quantitative analysis method with high-performance lamina chromatography (HPTLC). The results indicated that the main component of thick celery flavonoids is apigenin which has the same spectrum and chromatogram characteristics with the same Rf value(0.65) as the standard sample by the high-performance silica gel board G60 and the outspread solvent (chloroform:methanol:water, 18:2.3:0.35). The standard curve regress equation is: y=15138x +7594.8, R2=0.993 with linearity scope: 0.332 ～0.996μg per spot. The precision RSD(n=5) is 1.33 %, and the average callback rate is 97.82%.

Key words: high-performance liquid chromatography (HPLC), high-performance lamina chromatography (HPTLC), apigenin, Rf value