Abstract: Objective:Comparing the different ways of enriching selenium(Se) for barley seedling and mensurating the Se content in the Se-enriched barley seedlings. Methods:Barley se-edlings were cutivated with water and enriched Se by soaking seeds with Se,cultivating after sprouting with Se and cultivating in the whole process with Se. The Se enriched in barley seedling was assayed by atomic fluorescence spectrometry. Results:The barley seedlings cultivated with Se-after sprouting grow well when Na2SeO3 concentration is below 50 mg/L. The Se content is the highest when adding Na2SeO3 after sprouting. The organic Se content and the total Se content of the barley seedling are as 8.05 times and 4.97 times as that of control respectively when Na2SeO3 concentration is 30 mg/L. The barley seedlings grow slowly when Se is added in the whole process. Conclusion:Eriching Se with 30 mg/L Na2SeO3 after sprouting is the best way.

Key words: barley seedling, selenium, content