FOOD SCIENCE ›› 2008, Vol. 29 ›› Issue (8): 258-260.

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Optimization of Microwave-extration Conditions of Antibacterial Components from Aspergillus niger

 LI  Zhu, YU  Jian-Ping, XIAO  Yang, LIANG  Zong-Qi, LI  Ying   

  1. 1.Institute of Fungus Resource,College of Life Science,Guizhou University,Guiyang 550025,China;2.Institute of Biochemistry and Nutrition,College of Life Science,Guizhou University,Guiyang 550025,China;3.Institute of Products Detection and Determination of Guizhou Province,Guiyang 550003,China
  • Online:2008-08-15 Published:2011-08-26

Abstract: Objective:To optimize the microvave-extraction procedure of antibacterial components from Aspergillus niger xj. Methods:Extraction procedure was screened by orthogonal test according to the inhibiting zones diameter,which was measured through filter paper method using Agrobacterium tumefaciens T-37 as indicator. Results:The optimized extraction conditions are as follows:distilled water 20 ml,solid-liquid ratio 1:200,and treating for 3 min with middle fire power of micro -wave. Conclusion :The inhibiting zone diameter reaches 40.86 mm. The procedure is very simple and feasible,which can be used as reference of production.

Key words: microvave-extraction, Aspergillus niger, orthogonal test, inhibiting zone