Improvement on Determination of Malachite Green in Fishery Products by HPLC with Fluorescence Detector

Abstract

Abstract: In the use of China national standard Determination of malachite green and gentian violet residues in fishery products-high performance liquid chromatography with fluorescence detector(GB/T20361-2006),the problems concerning pretreatment,purification and detection were analyzed. The reduction effect of KBH4 was studied. Centrifugation was adopted for separation instead of separatory funnel in liquid-liquid extraction process. Meanwhile some corresponding operations were simplified. The determination results of tilapia,shrimp and eel by the improved method showed that the recoveries range from 80.7% to 109.8% with the relative standard deviations(RSD) from 0.967% to 9.169%.

Key words: malachite green, fluorescence detection, experimental conditions

YANG Xian-Qing, SUN Man-Yi, LI Lai-Hao, CEN Jian-Wei, WU Yan-Yan, QI Bo, SHI Hong. Improvement on Determination of Malachite Green in Fishery Products by HPLC with Fluorescence Detector[J]. FOOD SCIENCE, 2008, 29(8): 526-529 https://doi.org/

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Improvement on Determination of Malachite Green in Fishery Products by HPLC with Fluorescence Detector

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