Abstract: A rapid capillary electrophoresis method was established for detecting water-soluble vitamins in mushroom powder.Effects of buffer solution and voltage on the separation of vitamins from mushroom powder were studied.The effective ingredients of the samples were purified and enriched by means of a solid-phase extraction step with a pre-conditioned C18 cartridge,and the compounds were eluted with pH4.2 HCl solution.VB1,VB2,VB6,VC and VPP could be separated on a 50 cm ×75 μm capillary column with 20 mmol/L borate buffer solution(pH8.72),at temperature 25 ℃ and electrophoresis voltage 20 kV,within 10 min,and the satisfied results were obtained with different characteristics including linearity,precision and stability.

Key words: mushroom powder, Capillary zone electrophoresis, water-soluble vitamins