Extraction, Purification and Preliminary Analysis of Eleagnus angustifolius L. Polysaccharide-2

Abstract

Abstract: The crude Eleagnus angustifolius L.polysaccharide was obtained by a series of steps of degreasing, hot water extraction, alcohol precipitation, trichloroacetic acid treatment and further purified four times Sevag deproteinization and decoloring. EAP-1, EAP-2 and EAP-3 were gained by DEAE-cellulose and dialysis method. Qualitative chemical reaction and UV analysis indicated that EAP-2 is an acid polysaccharide with 2.3% D-galacturonic acid and without any protein, nuclear acid and starch. The units of the neutral monosaccharide are identified by HPLC as rhanmose, xylose, arabinose, fructose, mannose, glucose and galactose. The polysaccharide structure of EAP-2 is identified by IR.

Key words: Eleagnus angustifolius L., polysaccharides, extraction and purification, preliminary analysis

WANG Qing-Ning, 吕Xing-Lian , PENG Yun-Shuo, WU Ji-Ling. Extraction, Purification and Preliminary Analysis of Eleagnus angustifolius L. Polysaccharide-2[J]. FOOD SCIENCE, 2007, 28(1): 43-46.

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Extraction, Purification and Preliminary Analysis of Eleagnus angustifolius L. Polysaccharide-2

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