Abstract:  Objective and Methods: To study the anti-tumor effects of exopolysaccharides of Phellinus igniarius(PIEP), the effects of PIEP on the proliferation of tumour cell lines were assayed in vitro with MTT colorimetric method. Micronucleus tests in mouse bonemarrow polychromatic erythrocyte (PCE) and sperm abnormalities tests were used to researrch antimutaiton induced by cyclophosph--amide(CP). The activities of superoxide dismulase (SOD) and the content of malondialdehyde(MDA) in serum and liver of traumatic mice were respectively assayed to study their effects on lipid peroxide. Results: PIEP can inhibit proliferation of tumour cell significantly: IC50 to HepG-2 and MCF- 7 were 194.42; and 261.56μg/ml respectively; at doses of 300, 600mg/kg bw, MN inhibition rate can reach 44.78% and 49. 63% respectively; in comparison with CP group, PIEP can reduce sperm abnormality frequency significantly (p< 0.01) and PIEP can increase the activity of SOD and decrease the content of MDA in serum and liver of traumatic mice distinctly. Conclusion: PIEP has significant anti-tumour activity and lipid peroxide resisting effects. It can also inhibit gene mutation of body cells and germ cells induced by CP.

Key words: Phellinus igniarius, exopolysaccharide, anti-tumour, micronucleus test, sperm abnormality, lipidperoxide