Determination of Malachite Green in Aquatic Products by HPLC- FLD

Abstract

Abstract: In this paper, a method for determining the total residue of malachite green in aquatic products was developed by high- performance liquid chromatography (HPLC) with fluorescence detection. Malachite green in sample was reduced to leucomalachite green. Being extracted by acetonitrile-buffer mixture, cleaned-up and condensed, leucomalachite green was determined on a Symmetry shieldTMRP18 column with acetonitrile-water as mobile phase, and monitored with fluorescence detector (λem=265 nm andλem=360 nm). The linear range for leucomalachite green is 1～160 ng/ml. The detection limit of the method according to the total weight of malachite green is 1.0 μg/kg. The recovery is in the range of 82.6%～92.6%.

Key words: high performance liquid chromatography, fluorescence, aquatic products, malachite green

WANG Jun, LI Jian-Ye. Determination of Malachite Green in Aquatic Products by HPLC- FLD[J]. FOOD SCIENCE, 2007, 28(7): 364-367 https://doi.org/

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Determination of Malachite Green in Aquatic Products by HPLC- FLD

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