Optimization of Liquid Fermentation Medium of Cordyceps taishanensis Mycelia and Determination of Contents of Their Effective Components

Abstract

Abstract: Taking mycelial dry weight as the index for investigation, the optimum carbon-nitrogen ratio (C/N ratio) in liquid fermentation medium of Cordyceps taishanensis is determined as 3.0 to 4.0 by single factor test. ThroughL₉(3⁴) orthogonal test, the best formula of the medium is determined as maize meal 3.0%, glucose 1.5%, soybean flour 1.0% and yeast paste 0.8%. The average maximum growth quantity of mycelium is 1.29 g/100 ml. Furthermore, the contents of adenosine, uridine, inosine and hypoxanthine from natural Cordyceps sinensis (Berk.) Sacc. mycelia and cultured Cordyceps taishanensis mycelia were determined by thin-layer chromatographic scanning repectively. The contents of these four components in Cordyceps taishanensis mycelia are as 8.25 times, 3.76 times, 11.8 times and 1.55 times as those of Cordyceps sinensis (Berk.) Sacc.respectively, much higher. The contents of these four components in the two samples are uridine > inosine > adenosine > hypoxanthine.

Key words: Cordyceps taishanensis mycelia, liquid fermentation, uridine, adenosine, inosine, hypoxanthine

ZHAO Xue-Mei, SU Yan-You, WANG Gui-Ling, MA Teng, BI Yan-Ping. Optimization of Liquid Fermentation Medium of Cordyceps taishanensis Mycelia and Determination of Contents of Their Effective Components[J]. FOOD SCIENCE, 2008, 29(9): 384-387.

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Optimization of Liquid Fermentation Medium of Cordyceps taishanensis Mycelia and Determination of Contents of Their Effective Components

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