Abstract:

Objective: To explore the anti-obesity effect of hypoxanthine (Hx) on nutritionally obese mice. Methods:
An obese mouse model was established successfully by feeding high-fat diet. The ICR mice were randomly divided
into six groups, namely, normal control, model control, positive control (L-carnitine, 100 mg/kg) and hypoxanthine
treatment groups (30, 60, and 90 mg/kg). After 4 weeks of oral administration, blood samples were collected and
centrifuged and then the mice were sacrificed. The contents of triglyceride, total cholesterol, leptin and adiponectin
in mouse serum were detected. The adipose tissues were made into paraffin section to observe the changes of adipose
cells by HE staining. The gene expression levels of leptin and adiponectin in mouse epididymal adipose tissue
were measured by real-time quantitative polymerase chain reaction (qRT-PCR). Results: The body weight of the
Hx treatment groups were significantly lower than that of the model group (P < 0.05); TC levels were significantly
lower than those of the model group (P < 0.05, P < 0.01). The gene expression levels of leptin and adiponectin in
the middle- and low-dose Hx groups and normal control were significantly different from those of the model group
(P < 0.05). Conclusion: The middle- and low-dose Hx groups had good anti-obesity effect, and the best effect was achieved
at the low dose.

Key words: obesity, hypoxanthine, slice, real-time quantitative polymerase chain reaction (qRT-PCR)