FOOD SCIENCE ›› 2005, Vol. 26 ›› Issue (1): 170-172.

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Determination of 10-HDA in Royal Jelly and Its Products by RP-HPLC

 LONG  Zhou-Xiong, WAN  Chun-Hua, WANG  Kai, LIU  Bo-Ping, HU  Hai-Shan   

  1. Jiangxi Analysing and Testing Institute
  • Online:2005-01-15 Published:2011-09-19

Abstract: The contents of 10-hydroxy-2-decenoic acid in royal jelly and its products were determinded by RP-HPLC. 10-HDA could be separated on C18 column by using CH3OH-H2O-H3PO4 ( 55:45:0.2, V/V/V) as the mobile phase at a flow-rate of 1.0ml/ min,Ethyl-P-hyaroxybenzoate was used as the internal standard and then detected at UV 210 nm ,This method was simple, rapid and accurate, the results were reproducible. The recoveries were 97.6%~101.8%.

Key words: RP-HPLC, royal jelly, 10-HDA