FOOD SCIENCE ›› 2007, Vol. 28 ›› Issue (11): 450-452.

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Determination of Pineapple Saccharides Components by High Performance Liquid Chromatography

ZHANG  Xiu-Mei, DU  Li-Qing, XIE  Jiang-Hui, CHEN  Jia-Ying, GONG  De-Qiang, LI  Wei-Cai   

  1. 1.South Subtropical Crop Research Institute, China Tropical Agricultural Science Institute, Zhanjiang 524091, China; 2.College of Horticulture, South China Agricultural University, Guangzhou 510642, China
  • Online:2007-11-15 Published:2011-11-22

Abstract: Determination of saccharides components in pineapple was studied by high performance liquid chromatography (HPLC). The HPLC column is Luna 5μm NH2 100A, 250 mm×4.6 mm, the mobile phase is CH3CN:H2O=70:30, the temperature of the column is about 35℃, and the detector is series 200 refractive index detector. The relative standard deviations are 0.52%~ 2.24%, and the recoveries 98.8%~100.3%, while the linear related coefficient is more than 0.9992. The results showed that the samples are separated completely. The method is proved to be simple, rapid and reliable to determine the saccharides components in pineapple.

Key words: HPLC, pineapple fruit, fructose, glucose, sucrose