Screening and Identification of A Cold-adapted Cellulase-producing Strain and Characterization of Cellulase

Abstract

Abstract: Strain Z6 producing extracellular cellulase was isolated from seawater and sea mud collected from the Gaogong island of Lianyungang seaside. Its cell was rod shaped and gram-negative, with capsule and non-sporeforming. The fit range of growth temperature was 4～35 ℃, and the most suitable one was 25 ℃. The optimum pH for its growth was at pH8. The most suitable concentration of NaCl was 3%. The preliminary identification indicated that the strain belonged to Pseudoalteromonas sp. according to its morphological, the physiological and biochemical properties and 16SrRNA sequence analysis. Sequencing and phylogenetic analysis of the 16S rRNA gene of strain Z6 indicated that the strain belonged to Pseudoalteromonas carrageenovora. No work on Pseudoalteromonas carrageenovora producing cold-adapted cellulase had been reported so far. The optimal temperature and optimal pH for the cellulase produced by strain Z6 were 30℃ and 8.0 respectively. The cellulase still kept high activity at 10 ℃. It might have a potential value of industrial application.

Key words: cold-adapted cellulase, Pseudoalteromonas carrageenovora, screening, identification, enzyme charac-terization

吕Ming-Sheng , 吕Feng-Xia , FANG Yao-Wei, LIU Shu, WANG Shu-Jun. Screening and Identification of A Cold-adapted Cellulase-producing Strain and Characterization of Cellulase[J]. FOOD SCIENCE, 2007, 28(12): 235-239.

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Screening and Identification of A Cold-adapted Cellulase-producing Strain and Characterization of Cellulase

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