RP-HPLC Analysis of 6,1’,6’-Tri-O-tritylsucrose-penta-acetate(TRISPA)—An Intermediate of Sucralose Synthesis

Abstract

Abstract: A method for analysis of 6,1’,6’-Tri-O-tritylsucrose-penta-acetate(TRISPA), an important intermediate in the process of sucralose synthesis, was developed by reversed phase high performance liquid chromatography(RP-HPLC). The column was Waters XTerraTM RP18 5μm, 3.9 ×150 mm. The mobile phase was acetonitrile: water (84:16). The flow rate was 0.6 ml/min. TRISPA was determined with UV detector at 234 nm. Good linearity is obtained within the ranges from 0.05 to 0. 8 mg/ml and the minimum limit of detection is 5 ng. The average samples recovery is 99.2% and the relative standard derivation of this method is 0.34%. The results showed that the method is simple, rapid, accurate and sensitive.

Key words: sucralose, TRISPA, RP-HPLC

QIU Wen-Long, WANG Zhong-Fu, HUANG Lin-Juan, GUO Yu-Na. RP-HPLC Analysis of 6,1’,6’-Tri-O-tritylsucrose-penta-acetate(TRISPA)—An Intermediate of Sucralose Synthesis[J]. FOOD SCIENCE, 2007, 28(12): 379-382.

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RP-HPLC Analysis of 6,1’,6’-Tri-O-tritylsucrose-penta-acetate(TRISPA)—An Intermediate of Sucralose Synthesis

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