FOOD SCIENCE ›› 2006, Vol. 27 ›› Issue (7): 224-227.

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Study on Analysis of Main Glycosides from Siraitia grosvenorii Fruits with High-performance Liquid Chromatography

 ZHANG  Yun-Zhu, QI  Xiang-Yang, CHEN  Wei-Jun, ZHANG  Li-Qin   

  1. 1.College of Food Science and Technology, Huazhong Agricultural University, Wuhan 430070, China; 2.Jiaxing College, Jiaxing 314000, China
  • Online:2006-07-15 Published:2011-09-29

Abstract: Objective: To study the use of HPLC to assay the content of mogroside V, 11-oxo-mogroside, mogroside IV andSiamenoside I in Siraitia grosvenorii fruits. Methods: The RP-HPLC was used to determine the content of mogrosides. The analysis was carried on C18 column. Results: The conditions to establish the HPLC method for determination of mogrosides were: eluting solvent, acetonitrile-water, 23:77 (V/V); flowrate, 1.0ml/min; detection, 210nm; temperature, ambient and sensitivity setting, 0.04AUFS. The method is simple, easy and of good linear range (0.5~13.75μg) with the correlationship over 0.99. The recovery is 94.46%~105.05%, and the RSD 1.40%~3.31%. Conclusion: The HPLC method can be used to determine the content of mogrosides in Siraitia grosvenorii and its products.

Key words: HPLC, mogrosides, Siraitia grosvenorii